The Department of biology at the Faculty of education for pure sciences at Karbala University discussed a master thesis entitled (immunological and histological study of female rats with induced polycystic ovaries and evaluation of the effect of treatment with green nanocomposite before and after infection). The discussion for the student (Ala Shaheen dari), under the supervision of Asst. Prof. Dr. Caesar Abdul Sajjad Mohammed, first supervisor, and Asst.Prof.Dr. Alaa Hussein Mahdi) second supervisor. The aim of the thesis was to prepare and diagnose the green nanocomplex of fresh asparagus stems and to study the effect of both fresh asparagus extract and fresh green asparagus nanocomplex on induced polycystic ovaries, improve biological efficacy and evaluate the bio-effectiveness of the extract. The study recommended to study the effect of the asparagus nanoparticle compound on the histological and functional side in laboratory animals to find out its toxic effect on other tissues such as the liver and kidney and places of accumulation after the compound finishes delivering treatment and the mechanism of disposal of these compounds, as well as to conduct other studies more on the histological and physiological aspects of the effects of drugs with side effects and try to load them on the nanoparticle compound after examination and diagnosis to ensure the success of its green synthesis. The student was awarded a distinction.