Discussion of a Master’s Thesis

The Department of biology at the College of Education for Pure Sciences discussed the master’s thesis for student (Nawal Hadi Mohsen), entitled (The effect of some plant extracts on inhibiting the growth of P. aeruginosa isolated from burn injury in Karbala and the role of some arthropods in their transmission), under the supervision of (Prof.Dr. Rafid Abbas Al-Issa) and Assistant Professor Dr. (Caesar Abdel-Sajjad Muhammad). The aim of the study was:

1- Isolation and identification of P. aeruginosa from burn patients
2- Detection of the ability of bacteria to produce biofilm
3- Investigating the effect of plant extracts of thyme, pomegranate peel and tecuma leaves.
4- Studying the role of arthropods in transmitting bacteria.
5- Detection of effective chemical aggregates in plant extracts.
As for the most prominent conclusions:
1- Isolates of P. aeruginosa showed high resistance to antibiotics, except for the antibiotic Imipenim, where the bacteria were sensitive to it.
2- P. aeruginosa has a strong, medium or weak biofilm, and the plant extracts used in the study affected in varying proportions depending on the strength of the biofilm.
3- The aqueous and alcoholic plant extracts of thyme, pomegranate peel, tecuma leaves and wormwood had a clear inhibitory activity against P. aeruginosa.
4- The aqueous and alcoholic tecuma leaf extract showed a clear inhibitory activity, and the aqueous extract of the plant gave the highest rates of inhibition against Pseudomonas aeruginosa bacteria.

While the recommendations were:
1- Isolation of the active compounds included in the composition of the extracts to determine the active compound.
2- Expanding the application of the use of plant extracts and conducting experiments on them.
3- Conducting studies on the plant extracts used in the study.

After the extensive scientific discussion and listening to the student’s defense of her thesis, the method of her research and her use of sources and references, the thesis was approved with the appreciation of the blessed distinction of the student and her supervisors, the College of Education for Pure Sciences and the University of Karbala, and for more creativity in the service of our beloved Iraq, the Iraq of science and scholars, and from God Almighty success and payment.