Discussion of a Master’s Thesis
The Department of Life Sciences at the College of Education for Pure Sciences / University of Karbala discussed the master thesis tagged (detection of hsp65,mpt64 genes in people infected with Mycobacterium tuberculosis), by student (Heba Salem Jawad), and under the supervision of a Prof. Hiam Abdel Reda Karim. Where the thesis aims to detect the hsp65,mpt64 genes in patients with MTB bacteria, and the goal is achieved by diagnosing pulmonary tuberculosis bacteria among the patients attending the Tuberculosis Center in the Holy Karbala Governorate, using the Acid fast stain method and the GeneXpert device, and investigating the two genes hsp65,mpt64 using PCR technology (polymerase chain). reaction), the relationships between smoking, environment, age and gender. The message showed that the infection rate of males in the urban environment was higher than females, and smoking is a risk factor for pulmonary tuberculosis and not a main cause, and smoking also has a relationship with the gender and category of the patient, where there was an increase in the rates of male smokers and the third group was the most smoking factor. Smoking between urban and rural patients did not register any significant differences, the percentage of infection of the second type Acide fast stain +2 was significantly higher in males compared to females, and the thesis showed that pulmonary tuberculosis is a highly virulent disease that affects adults, the elderly and both sexes.
The researcher recommended conducting molecular studies of other genes related to pulmonary tuberculosis, and studying the effect of infection with respiratory viruses such as COVID-19 virus in people with pulmonary tuberculosis, revealing the effect of some immunological parameters when infected with pulmonary tuberculosis, and making anti-tuberculosis preventive treatment available to the population most at risk. Infection with the disease, the use of the polymerase chain reaction test or the immunoassay to detect infection with pulmonary tuberculosis, and the study of the relationship of the mpt64 gene with other types and strains related to pulmonary tuberculosis bacteria. The student received an honors grade.
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