assistant teacher Hussein Ali Ahmed from Department of Chemistry at the College of Education for Pure Sciences, obtained a publication of his research entitled (Preparation, characterization, and study of antimicrobials and toxicology studies of new phenylenediamine derivatives – Formazan derivatives) in the international scientific journal (Indian Journal of Forensic Medicine and Toxicology). Scientific journals included in the Scopus classification group of international scientific journals. The aim of the research was to use formazans as a medicine and antibiotics for cancerous tumors, as formazans are characterized by containing highly effective functional groups that have an effect on the possession of any compound that has a pharmaceutical and biological property such as antimicrobials for many microbes, as these compounds have proven high resistance to the spread and spread of cancerous tumors. Bacteria and fungi. The formasan derivative chain was synthesized in this study from methphenylene dimine through an imitation reaction and then a coupling reaction to produce five new derivatives. Formazan derivatives were examined by different spectroscopic techniques represented by (infrared – infrared spectra, H. NMR – proton magnetic resonance, mass – spectra) – as spectroscopic measurements, then a biological study through different biological experiments in this field such as toxicity Cellular, anti-bacterial resistance.